1. Effects of olive oil polyphenols on erythrocyte oxidative damage.
In this work, Pavla-Martins et al.. studied the capacity of oleacein to protect red blood cells (RBCs) from oxidative injury. The in vitro oxidative stress of RBCs was induced by the water-soluble radical initiator 2,2'azobis (2amidinopropane) dihydrochloride and changes were evaluated either by optical microscopy or by the amount of hemolysis. Oleacein was shown to significantly protect RBCs from oxidative damage in a dose-dependent manner. Oleacein had the most powerful effect at 20mM, within the other polyphenols. Even at 3mM, oleacein still had an important protective activity. For the first time it was demonstrated that oleacine may play a noteworthy protective role against ROS-induced oxidative injury in human cells since lower doses of this compound were needed to protect RBCs in vitro from oxidative mediated hemolysis.
Paiva-Martins F et al.., Mol Nutr Food Res. 2009
Oleacein protects red blood cells (RBCs)from oxidative injury
In this work, Pavla-Martins et al.. studied the capacity of oleacein to protect red blood cells (RBCs) from oxidative injury. Oleacein was shown to significantly protect RBCs from oxidative damage in a dose-dependent manner. Even at low dosages exhibited a remarkable protective role for RBCs.
Oleacein protects red blood cells from oxidative mediated hemolysis,even at low dosages.
2. Oleacein. Translation from Mediterranean Diet to Potential Antiatherosclerotic Drug
Oleacein, due to its abundance, in olive oil, it may play a special role in decreasing the progression of atherosclerosis. Some bioactivities of oleacein, such as antioxidant, anti-inflammatory, anti-proliferative and antimicrobial, were documented. There is also evidence of the bioavailability of oleacein in humans as well. However, due to the lack of clinical data, further studies are needed to provide information about the usefulness of this compound in antiatherosclerotic therapy.
Oleacein:a potential Antiatherosclerotic Drug
Oleacein may play a special role in decreasing the progression of atherosclerosis, the most important cause of stroke or heart attack. Some bioactivities of oleacein have been already documented, further studies are needed about the usefulness of this compound in antiatherosclerotic therapy.
Oleacein plays a special role in decreasing the progression of atherosclerosis, the mostimportant causeof strokeor heart attack.
3. Oleacein enhances anti-inflammatory activity of human macrophagesby increasing CD163 receptor expression.
Filipek et al.. examined whether oleacein could increase CD163 and IL10 receptor expression as well as intracellular secretion of protein heme oxygenase 1 (HO1) in human macrophages. Effect of oleacein (10 and 20 μmol/l) or oleacein together with complexes of haemoglobin (Hb) and haptoglobin 11 (Hp11) or haptoglobin 22 (Hp22) on expression of IL10 and CD163 receptors was determined by Flow Cytometry. HO1 intracellular secretion in macrophages was investigated by enzyme-linked immunosorbent assay (ELISA). Oleacein together with complexes HbHp11 or HbHp22 stimulated the expression of CD163 (30-100 fold), IL10 (170-300 fold) and HO1 secretion (60-130 fold) after 5 days of co-incubation. Our results suggested that oleacein enhances anti-inflammatory activity of complexes haemoglobin with haptoglobin 11 and 22 and could play a potential role in the prevention of inflammatory disease related to atherosclerosis.
The anti-inflammatory effect of oleacein and mechanisms of action
Filipek et al. examined the ability of oleacein to increase the production of specific anti-inflammatory proteins. In particular, oleacein is either associated with specific blood components and induces the production of some anti-inflammatory proteins or inflammatory-related protein. Thus it seems that oleacein may play an important role in the prevention of inflammatory disease related to atherosclerosis.
Oleacein enhancesanti-inflammatory activity of specificcomplexesand could play a potential role in the prevention of inflammatory disease related to atherosclerosis.
4. Oleuropein and oleacein may restore biological functions of endothelial progenitor cells impaired by angiotensin II via activation of Nrf2/heme oxygenase1pathway.
Oleacein was examined if is able to protect Endothelial progenitor cells EPCs against impairment of their functions due to angiotensin-induced cell senescence. CD31(+)/VEGFR2(+) cells were cultured with angiotensin in presence or absence of increasing concentrations (from 1.0 to 10.0 μM) of oleacein. As compared to angiotensin II-treated cells, EPCs exposed to oleacein prior to angiotensin II showed a significant increase of proliferation and telomerase activity, and a decrease in the percentage of senescent cells and intracellular ROS formation. Oleacein restored migration, adhesion and tube formation of EPCs diminished by angiotensin II in a concentration-dependent manner. This effect was related to NFE2-related factor 2 (Nrf2) transcription factor activation and the increase of heme oxygenase1 (HO1) expression.
Oleacein protects cellsfrom aging
Oleacein was examined if is able to protect cells from aging and hence from their death. Endothelial progenitor cells exposed to oleacein showed a significant increase of proliferation in a dose-dependent manner.
Oleacein protects cellsfrom aging and death in a dose-dependent manner.
5. One-step semisynthesisof oleacein and the determination as a 5-lipoxygenaseinhibitor.
5-lipoxygenase is a direct target for oleacein with an inhibitory potential (IC50: 2 μM) more potent than oleocanthal and oleuropein. This enzyme catalyzes the initial steps in the biosynthesis of pro-inflammatory leukotrienes. This investigation presented here an alternative solution to isolation or total synthesis for the procurement of oleacein, thus facilitating the further development as a potential anti-inflammatory agent.
Vougogiannopoulou K et al., J Nat Prod. 2014
6. Oleacein may inhibit destabilization of carotid plaques from hypertensive patients. Impact on high mobility group protein-1.
The aim this study was to investigate a potential role of oleacein in attenuation of carotid plaque destabilization ex vivo. Oleacein at the concentrations of 10 and 20 μM significantly (P < 0.001) decreased secretion of HMGB1 (up 90%), MMP-9 (up to 80%) proteins, MMP-9/NGAL complex (up to 80%) and TF protein (more than 90%) from the treated plaque, as compared to control. At the same time IL-10 and HO-1 release increased by more than 80% (P < 0.001). Those results indicate that oleacein possess ability to attenuate the destabilization of carotid plaque and could be potentially useful in the reduction of ischemic stroke risk.
Oleacein reduces the ischemic stroke risk.
Oleacein has an effect against the destabilization of carotid plaques, by affecting the production of specific proteins associated with atherosclerosis. Ex vivo experiments showed that oleacein has the potential to attenuate the destabilization of carotid plaques and may be useful in reducing the risk of ischemic stroke.
Οleacein possessability to attenuate the destabilization of carotid plaque and couldbe potentially useful in the reduction of ischemicstroke risk.
Phytomedicine. 2017 Aug 15;32:68-73. doi: 10.1016/j.phymed.2017.06.004. Epub 2017 Jun 13.Filipek A1, Czerwińska ME1, Kiss AK1, Polański JA2, Naruszewicz M3.